MGB Eclipse® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization, while the Eclipse® has a broad absorption range that quenches many of the most common fluorophores, including FAM, HEX, TET and Yakima Yellow®.
An NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides, as well as peptides or proteins. NHS esters are especially useful in situations where the equivalent phosphoramidites exhibit instability to the conditions of oligonucleotide synthesis and/or deprotection.
Glen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the sequence. The resultant fluorescent oligonucleotide probes are typically used in a wide variety of applications like genetic and genome analyses, and forensic analysis. Common applications of fluorescent oligonucleotide probes are FRET and Molecular Beacon probes.